---
title: Evaluation of the Modified Two-Tiered Testing Method for Diagnosis of Lyme Disease in Children
description: "Lyme disease research: MTTT has shown promising results as alternative strategy for diagnosis of Lyme disease in adults but yet to be evaluated in children."
---

<https://www.globallymealliance.org/research>

# [Evaluation of the Modified Two-Tiered Testing Method for Diagnosis of Lyme Disease in Children](https://www.globallymealliance.org/research/evaluation-of-the-modified-two-tiered-testing-method-for-diagnosis-of-lyme-disease-in-children)

 Written by [Global Lyme Alliance](https://www.globallymealliance.org/research/author/glalyme) | Jan 28, 2020 6:25:39 PM

Conventional two-tiered testing (CTTT) for Lyme disease includes a first-tier enzyme immunoassay (EIA) followed by a supplemental immunoblot, and modified two-tiered testing (MTTT) relies on two different sequential EIAs without the inclusion of an immunoblot. MTTT has shown promising results as an alternative strategy for the diagnosis of Lyme disease in adults but has not yet been evaluated in children. We performed a cross-sectional study of children and adolescents ≤21 years of age undergoing clinical investigation for suspected Lyme disease.

Published in *Journal of Clinical Microbiology*: [https://jcm.asm.org/content/57/10/e00547-19](https://jcm.asm.org/content/57/10/e00547-19)  
 PubMed: [https://www.ncbi.nlm.nih.gov/pubmed/31413078](https://www.ncbi.nlm.nih.gov/pubmed/31413078)

[View full post](https://www.globallymealliance.org/research/evaluation-of-the-modified-two-tiered-testing-method-for-diagnosis-of-lyme-disease-in-children)

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